Optimal selection of epitopes for TXP-immunoaffinity mass spectrometry
نویسندگان
چکیده
منابع مشابه
Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry.
BACKGROUND Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable method for high-throughput clinical applications. Mass spectrometry (MS) can in ...
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Protein methylation is a common posttranslational modification that mostly occurs on arginine and lysine residues. Arginine methylation has been reported to regulate RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation is best known to regulate histone function and is involved in epigenetic regulation of gene transcription. To...
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Both ligand binding assays (LBA) and mass spectrometry (MS) methods are widely used in routine clinical analysis. In recent years, an interesting trend has emerged which blends these 2 techniques to combine the selectivity of immunoaffinity (IA) extraction with the specificity of MS detection (IA-MS). The article by Laha and coworkers in the current issue of Clinical Chemistry, entitled “Charac...
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Strategies for removal of high abundance proteins have been increasingly utilized in proteomic studies of serum/plasma and other body fluids to enhance the detection of low abundance proteins and achieve broader proteome coverage; however, both the reproducibility and specificity of the high abundance protein depletion process still represent common concerns. Here we report a detailed evaluatio...
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BACKGROUND Quantification of serum tumor markers plays an important role in determining whether patients treated for cancer require further therapy. Whereas large-scale proteomic efforts aim to identify novel tumor markers to facilitate early detection, optimization of methods for quantifying known tumor markers offers another approach to improving management of malignancies. For example, immun...
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ژورنال
عنوان ژورنال: Algorithms for Molecular Biology
سال: 2010
ISSN: 1748-7188
DOI: 10.1186/1748-7188-5-28